Engineering and analysis of load bearing tissues with heterogeneous cell populations are still a challenge. Here, we describe a method for creating bi-layered alginate hydrogel discs as a platform for co-culture of diverse cell populations within one construct.
Sharma, P., Twomey, J. D., Patkin, M., Hsieh, A. H. Layered Alginate Constructs: A Platform for Co-culture of Heterogeneous Cell Populations. J. Vis. Exp. (114), e54380, doi:10.3791/54380 (2016).