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JoVE Journal
Biochemistry

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Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
 

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Article DOI: 10.3791/54391
January 16th, 2017

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Summary January 16th, 2017

This manuscript describes an approach to isolate select cognate RNPs formed in eukaryotic cells via a specific oligonucleotide-directed enrichment. We demonstrate the applicability of this approach by isolating a cognate RNP bound to the retroviral 5' untranslated region that is composed of DHX9/RNA helicase A.

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