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JoVE Journal
Biology

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Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry
 

Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry

Article doi: 10.3791/54416
August 16th, 2016

Summary August 16th, 2016

Herein we describe a sensitive immunochemical method for mapping the spatial distribution of 5mC oxidation derivatives based on the use of peroxidase-conjugated secondary antibodies and tyramide signal amplification.

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