Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(<em>N</em>-isopropylacrylamide) Microgels

Otto L. J. Virtanen; Ashvini Purohit; Monia Brugnoni; Dominik W&#246;ll; Walter Richtering

doi:10.3791/54419

Journal

/

Chemistry

/

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(<em>N</em>-isopropylacrylamide) Microgels

JoVE Journal
Chemistry

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Chemistry

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

10,221 Views

•

11:34 min

•

September 08, 2016

DOI:

10.3791/54419-v

DOI:

10.3791/54419-v

•

11:34 min

September 08, 2016

•

10195 Views

Otto L. J. Virtanen¹, Ashvini Purohit¹, Monia Brugnoni¹, Dominik Wöll¹, Walter Richtering¹

¹Institute of Physical Chemistry,RWTH Aachen University

Summary

Non-stirred precipitation polymerization provides a rapid, reproducible prototyping approach to the synthesis of stimuli-sensitive poly(N-isopropylacrylamide) microgels of narrow size distribution. In this protocol synthesis, light scattering characterization and single particle fluorescence tracking of these microgels in a wide-field microscopy setup are demonstrated.