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Capture and Release of Viable Circulating Tumor Cells from Blood
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Cancer Research
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JoVE Journal Cancer Research
Capture and Release of Viable Circulating Tumor Cells from Blood

Capture and Release of Viable Circulating Tumor Cells from Blood

DOI:
10.3791/54435-v

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08:10 min

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October 28, 2016

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, ,
1Department of Pathology,University of Miami, 2SRI International, 3Department of Biomedical Engineering, DJTMF Biomedical Nanotechnology Institute at the University of Miami,University of Miami

Chapters

  • 00:05Title
  • 00:59Coating the Microfilter
  • 02:23Filtration Cassette Assembly
  • 03:14Filtration of the Blood Sample
  • 06:17Results: Effective Capture, Release, and Retrieval of Viable CTCs from Blood
  • 07:33Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol to utilize a poly(N-iso-propylacrylamide) (PIPAAm) coated microfilter for effective capture and thermoresponsive release of viable circulating tumor cells (CTC) is presented. This method allows capture of CTC from patients' blood and subsequent release of viable CTC for downstream off-chip culture, analyses and characterization.

Tags

Keywords: Circulating Tumor CellsCTCsLabel-free CaptureViable CTCsMetastasisDisease ProgressionResponse To TherapyRecurrence RiskPIPAAmMicrofilterFiltration CassetteSpin Coater

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