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JoVE Journal
Cancer Research

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Capture and Release of Viable Circulating Tumor Cells from Blood
 

Capture and Release of Viable Circulating Tumor Cells from Blood

Article doi: 10.3791/54435
October 28th, 2016

Summary October 28th, 2016

A protocol to utilize a poly(N-iso-propylacrylamide) (PIPAAm) coated microfilter for effective capture and thermoresponsive release of viable circulating tumor cells (CTC) is presented. This method allows capture of CTC from patients' blood and subsequent release of viable CTC for downstream off-chip culture, analyses and characterization.

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