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Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications

Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications

DOI:
10.3791/54462-v

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10:45 min

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September 29, 2016

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,
1Department of Chemical and Biomolecular Engineering,University of Delaware, 2Department of Materials Science and Engineering,University of Delaware

Chapters

  • 00:05Title
  • 00:48Hydrogel Formation and Photopatterning
  • 03:46Visualizing and Quantification of Photopatterning
  • 06:18Encapsulating Cells and Photopatterning in the Presence of Cells
  • 07:16Determining the Viability and Metabolic Activity of Encapsulated Cells
  • 08:21Results: Quantitative and Qualitative Assessment of Photopatterning and Cell Viability
  • 09:44Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a sequential process for light-mediated formation and subsequent biochemical patterning of synthetic hydrogel matrices for three-dimensional cell culture applications. The construction and modification of hydrogels with cytocompatible photoclick chemistry is demonstrated. Additionally, facile techniques to quantify and observe patterns and determine cell viability within these hydrogels are presented.

Tags

Keywords: HydrogelCell CultureLight-mediatedPatterningSynthetic Extracellular MatrixIn VitroCell EncapsulationDrug DeliveryPolymerizationModulusSyringe Tip MoldGlass Slide Mold

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