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JoVE Journal
Bioengineering

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Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications
 

Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications

Article DOI: 10.3791/54462
September 29th, 2016

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Summary September 29th, 2016

We describe a sequential process for light-mediated formation and subsequent biochemical patterning of synthetic hydrogel matrices for three-dimensional cell culture applications. The construction and modification of hydrogels with cytocompatible photoclick chemistry is demonstrated. Additionally, facile techniques to quantify and observe patterns and determine cell viability within these hydrogels are presented.

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