Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes

The overall goal of this procedure is to effectively deliver solutions into the cytoplasm of livestock zygotes using a blunt-end needle with the aid of a laser. This method is used in research and in the animal trans-genesis field, since one is associated with a genome-editing tool, such as a CRISPR Cas9 system, it can create new cows to study gene function and transgenic animals in a very simple and reliable way. The main advantage of this technique is that it can be used to deliver any solution into a cytoplasm of large animal's zygotes.

With a handy person's injection efficiency minimalizes animal liver lomotil rates. First, prepare the injection micropipette by placing a borosilicate glass capillary into a micropipette puller and locking it into place. Use an appropriate program to pull the capillary so it results in a thin tip with a long taper.