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JoVE Journal
Genetics

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Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
 

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Article doi: 10.3791/54473
October 9th, 2016

Summary October 9th, 2016

Tandem splicing events occur at sites less than 12 nucleotides apart. Quantifying ratios of such splice variants is feasible using an absolute quantitative PCR approach. This manuscript describes how splice variants of the gene STAT3, in which two splicing events results in Serine-701 inclusion/exclusion and α/β C-termini, can be quantified.

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