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JoVE Journal
Biochemistry

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Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
 

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues

Article DOI: 10.3791/54485
February 17th, 2017

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Summary February 17th, 2017

Trehalose analogues are emerging as important molecules for bio(techno)logical and biomedical applications. We describe an optimized protocol for enzymatically synthesizing and purifying trehalose analogues that is simple, efficient, fast, and environmentally friendly. Its application to the rapid production and administration of a probe for the detection of mycobacteria is demonstrated.

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