Journal
/
Chemistry
/
Spin Saturation Transfer Difference NMR (SSTD NMR): A New Tool to Obtain Kinetic Parameters of Chemical Exchange Processes
JoVE Journal
Chemistry
This content is Free Access.
JoVE Journal Chemistry
Spin Saturation Transfer Difference NMR (SSTD NMR): A New Tool to Obtain Kinetic Parameters of Chemical Exchange Processes

Spin Saturation Transfer Difference NMR (SSTD NMR): A New Tool to Obtain Kinetic Parameters of Chemical Exchange Processes

DOI:
10.3791/54499-v

•

11:44 min

•

November 12, 2016

•

, , ,
1School of Chemistry,University of East Anglia, 2School of Pharmacy,University of East Anglia

Chapters

  • 00:05Title
  • 01:09Nuclear Magnetic Resonance (NMR) Sample Preparation and Experimental Setup
  • 03:50SSTD NMR Experiment Acquisition
  • 06:42SSTD NMR Experiment Processing
  • 08:53Results: Calculation of Kinetic Parameters in the Rotation of the Amide Bond of N,N-dimethylacetamide via the SSTD NMR Technique
  • 10:28Conclusion

Summary

Automatic Translation

Automatic Translation

A detailed protocol describing the SSTD NMR method is presented here to help new users apply this new method to obtain the kinetic parameters of their own systems undergoing chemical exchange.

Tags

Keywords: Spin Saturation Transfer Difference NMR (SSTD NMR)Chemical ExchangeKinetic ParametersMolecule DynamicsOrganic MoleculesOrganometallic MoleculesRotational VarianceConformational EquilibriumNitrogen InversionLignin ExchangeTautomerizationTemperature ControlProton NMRSTDDIFF Pulse Sequence90 Degree Hard Pulse

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/54499?article_type=v

Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development
check_url/54499?article_type=v

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
check_url/54499?article_type=v

Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins
check_url/54499?article_type=v

Ethylene Polymerizations Using Parallel Pressure Reactors and a Kinetic Analysis of Chain Transfer Polymerization
check_url/54499?article_type=v

Highly Stereoselective Synthesis of 1,6-Ketoesters Mediated by Ionic Liquids: A Three-component Reaction Enabling Rapid Access to a New Class of Low Molecular Weight Gelators
check_url/54499?article_type=v

Synthesis and Exfoliation of Discotic Zirconium Phosphates to Obtain Colloidal Liquid Crystals
check_url/54499?article_type=v

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
check_url/54499?article_type=v

NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements
check_url/54499?article_type=v

Application and Methodology of the Non-destructive 19F Time-domain NMR Technique to Measure the Content in Fluorine-containing Drug Products
check_url/54499?article_type=v

A New Straightforward Method for Lipophilicity (logP) Measurement using 19F NMR Spectroscopy

Read Article