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A Non-invasive and Technically Non-intensive Method for Induction and Phenotyping of Experimental Bacterial Pneumonia in Mice
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Non-invasive and Technically Non-intensive Method for Induction and Phenotyping of Experimental Bacterial Pneumonia in Mice

A Non-invasive and Technically Non-intensive Method for Induction and Phenotyping of Experimental Bacterial Pneumonia in Mice

DOI:
10.3791/54508-v

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07:43 min

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September 28, 2016

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1Immunity, Inflammation and Disease Laboratory, National Institute of Environmental Health Sciences (NIEHS),National Institutes of Health

Chapters

  • 00:05Title
  • 00:53K. pneumoniae Culture Preparation
  • 02:36Murine Intratracheal (i.t.) K. pneumoniae Aspiration
  • 03:26Bronchoalveolar Lavage Fluid (BALF) Collection and Analysis
  • 04:23Bacterial Lung and Peripheral Tissue Load Determination
  • 05:20Results: Representative Murine Systemic and Immune Cell Responses to K. pneumoniae Oropharynx Delivery
  • 06:42Conclusion

Summary

Automatic Translation

Automatic Translation

Several methods have been described in the literature for modeling bacterial pneumonia in mice. Herein, we describe a non-invasive, inexpensive, rapid method for inducing pneumonia via aspiration (i.e., inhalation) of a bacterial inoculum pipetted into the oropharynx. Downstream methods for assessment of the pulmonary innate immune response are also detailed.

Tags

Keywords: Bacterial PneumoniaMouse ModelNon-invasiveKlebsiella PneumoniaeInnate Immune ResponsePulmonary InfectionInfectious DiseaseImmunologyBiosafety Level 2Serial DilutionsOptical DensityCFUIntranasal Inoculation

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