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JoVE Journal
Genetics

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In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining
 

In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining

Article doi: 10.3791/54516
October 15th, 2016

Summary October 15th, 2016

Drosophila oogenesis continues to be exceptionally useful in the study of mitochondrial proliferation and inheritance. This manuscript describes a detailed protocol used to label the replicating mitochondrial DNA (mtDNA) in Drosophila adult ovaries with 5-ethynyl-2´-deoxyuridine (EdU), which facilitates uncovering mechanisms associated with mitochondrial inheritance that were previously debatable.

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