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Protein Complex Affinity Capture from Cryomilled Mammalian Cells
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Protein Complex Affinity Capture from Cryomilled Mammalian Cells

Protein Complex Affinity Capture from Cryomilled Mammalian Cells

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10:37 min

December 09, 2016

DOI:

10:37 min
December 09, 2016

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Summary

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Here we describe protocols to disrupt mammalian cells by solid-state milling at a cryogenic temperature, produce a cell extract from the resulting cell powder, and isolate protein complexes of interest by affinity capture upon antibody-coupled micron-scale paramagnetic beads.

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