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JoVE Journal
Genetics

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Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
 

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

Article DOI: 10.3791/54522 11:42 min September 30th, 2016
September 30th, 2016

Chapters

Summary

We describe a robust gene replacement strategy to genetically manipulate the smut fungus Ustilago maydis. This protocol explains how to generate deletion mutants to investigate infection phenotypes. It can be extended to modify genes in any desired way, e.g., by adding a sequence encoding a fluorescent protein tag.

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Tags

Genetic Manipulation Plant Pathogen Ustilago Maydis Fungal Biology Plant-microbe Interactions Strain Generation Method Functional Characterization Gene Deletion Promoter Exchange Fusion Constructs Phenotype Verification Protoplasting Reaction Preculture Inoculation YEPS-Light Exponential Phase Growth Optical Density Measurement Contamination Identification
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