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Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata
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Genetics
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JoVE Journal Genetics
Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata

Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata

DOI:
10.3791/54528-v

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09:45 min

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October 01, 2016

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,
1Department of Biology and Biotechnology,University of Pavia

Chapters

  • 00:05Title
  • 00:37Maintaining Medfly Colonies
  • 02:58Microinjection of Medfly Embryos
  • 06:34Screening for Transformants
  • 08:10Results: Direct Functional Characterization of a Gene and Generation of a Transgenic Strain
  • 09:06Conclusion

Summary

Automatic Translation

Automatic Translation

The Mediterranean fruit fly (medfly) Ceratitis capitata (Diptera: Tephritidae) is a worldwide pest of agriculture. A deeper understanding of its biology is key to control medfly populations and thus reduce economic impact. Embryo microinjection is a fundamental tool allowing both germ-line transformation and reverse genetics studies in this species.

Tags

Keywords: Ceratitis CapitataEmbryo MicroinjectionNucleic Acid DeliveryAgricultural PestTransgenic StrainsMedfly ColonyLarval FoodPupationMicro-injectionEmbryo Collection

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