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JoVE Journal
Immunology and Infection

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Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses
 

Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses

Article doi: 10.3791/54554
November 16th, 2016

Summary November 16th, 2016

This protocol describes how to inoculate C57BL/6J mice with the EGD strain of Listeria monocytogenes (L. monocytogenes) and to measure interferon-γ (IFN-γ) responses by natural killer (NK) cells, natural killer T (NKT) cells, and adaptive T lymphocytes post-infection. This protocol also describes how to conduct survival studies in mice after infection with a modified LD50 dose of the pathogen.

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