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Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses
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Immunology and Infection
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JoVE Journal Immunology and Infection
Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses

Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses

DOI:
10.3791/54554-v

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10:10 min

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November 16, 2016

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, , , ,
1Department of Immunology,University of Toronto, 2Toronto General Research Institute,University Health Network, 3Women’s College Research Institute

Chapters

  • 00:05Title
  • 00:54Glycerol Stock Long-term Storage Preparation
  • 02:18Experimental L. monocytogenes Inoculum Preparation and Infection
  • 03:44Peak Infection Spleen and Liver Bacterial Load Measurement
  • 06:16Effects of L. monocytogenes on CD4+ and CD8+ Cell IFN-gamma Responses
  • 07:43Results: Representative NK, NKT, and CD4 and CD8 T cell IFN-gamma Responses to L. monocytogenes
  • 08:54Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes how to inoculate C57BL/6J mice with the EGD strain of Listeria monocytogenes (L. monocytogenes) and to measure interferon-γ (IFN-γ) responses by natural killer (NK) cells, natural killer T (NKT) cells, and adaptive T lymphocytes post-infection. This protocol also describes how to conduct survival studies in mice after infection with a modified LD50 dose of the pathogen.

Tags

Keywords: Listeria MonocytogenesExperimental InfectionInterferon GammaC57 Black MiceBacterial LoadSurvivalBHI AgarBHI BrothOD600PBSCentrifugationDilution

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