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Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format

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Biology

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Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format

Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format

DOI:

10.3791/54584-v

•

08:54 min

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September 17, 2016

•

Scott M. Paulissen, Linda S. Huang

¹Department of Biology,University of Massachusetts Boston

Chapters

00:05Title
00:54Preparing Cells of the Same Genotype for Sporulation
02:55Preparing Cells of Different Genotypes for Sporulation
04:42Sporulating Cells in a 96-multiwell Format
06:29Results: Efficient Sporulation in a Multiwell Plate Format
08:05Conclusion

Summary

Automatic Translation

Here, sporulation of Saccharomyces cerevisiae is carried out in a 96 multiwell format.

Tags

Keywords: Sporulation Saccharomyces Cerevisiae 96-well Format High-throughput Screening Meiosis Tiled Two Micron Plasmid Library High Copy Suppressors Refractile Spores Aeration SK1 Yeast Strain YPG Plate YPD Plate YPD Culture YPA Culture Centrifugation Sporulation Media 96 Multiwell Plate

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