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JoVE Journal
Immunology and Infection

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Isolation and Activation of Murine Lymphocytes

Isolation and Activation of Murine Lymphocytes

Article DOI: 10.3791/54596
October 30th, 2016


Summary October 30th, 2016

Lymphocytes are the major players in adaptive immune responses. Here, we present a lymphocyte purification protocol to determine the physiological functions of the desired molecules in lymphocyte activation in vitro and in vivo. The described experimental procedures are suitable for comparing functional capacities between control and genetically modified lymphocytes.


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