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JoVE Journal
Biochemistry

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Defining Substrate Specificities for Lipase and Phospholipase Candidates
 

Defining Substrate Specificities for Lipase and Phospholipase Candidates

Article DOI: 10.3791/54613
November 23rd, 2016

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Summary November 23rd, 2016

Many predicted (phospho)lipases are poorly characterized with regard to their substrate specificities and physiological functions. Here we provide a protocol to optimize enzyme activities, search for natural substrates, and propose physiological functions for these enzymes.

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