Journal
/
Biology
/
In Vivo Study of Human Endothelial-Pericyte Interaction Using the Matrix Gel Plug Assay in Mouse
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
In Vivo Study of Human Endothelial-Pericyte Interaction Using the Matrix Gel Plug Assay in Mouse

In Vivo Study of Human Endothelial-Pericyte Interaction Using the Matrix Gel Plug Assay in Mouse

DOI:
10.3791/54617-v

•

08:16 min

•

December 19, 2016

•

, , ,
1Division of Pulmonary and Critical Care Medicine, School of Medicine,Stanford University, 2Stanford Cardiovascular Institute, School of Medicine,Stanford University, 3VA Palo Alto Health Care System, Department of Cardiothoracic Surgery, School of Medicine,Stanford University

Chapters

  • 00:05Title
  • 00:56Cell and Matrix Gel Preparation
  • 03:24Matrix Gel Injection
  • 05:05Plug Isolation: 14 Days Post-injection
  • 06:16Result: H&E and Immunofluorescent Staining
  • 07:00Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol to study human endothelial-pericyte interactions in mouse using a variation of the matrix gel plug angiogenesis assay.

Tags

Keywords: In VivoEndothelial-pericyte InteractionMatrix Gel Plug AssayMouseAngiogenesisBlood Vessel FormationSignaling PathwaysVascular DisordersEndothelial CellsPericytesCell CultureMatrigelBeta-FGFTrypsinizationCell CountingCell Injection

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/54617?article_type=v

Optimized Fibrin Gel Bead Assay for the Study of Angiogenesis
check_url/54617?article_type=v

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer
check_url/54617?article_type=v

Clonogenic Assay: Adherent Cells
check_url/54617?article_type=v

Do-It-Yourself Device for Recovery of Cryopreserved Samples Accidentally Dropped into Cryogenic Storage Tanks
check_url/54617?article_type=v

In Vivo Modeling of the Morbid Human Genome using Danio rerio
check_url/54617?article_type=v

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism
check_url/54617?article_type=v

Measuring the Stiffness of Ex Vivo Mouse Aortas Using Atomic Force Microscopy
check_url/54617?article_type=v

Sample Preparation for Rapid Lipid Analysis in Drosophila Brain Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
check_url/54617?article_type=v

Exploring Cellular Zinc Regulation Through ZnT1 Functionality (Video) | JoVE
check_url/54617?article_type=v

Author Spotlight: Investigating Angiogenesis and Vessel Permeability Through a Modified Matrix Gel Plug Assay

Read Article