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Application of Impermeable Barriers Combined with Candidate Factor Soaked Beads to Study Inductive Signals in the Chick
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Application of Impermeable Barriers Combined with Candidate Factor Soaked Beads to Study Inductive Signals in the Chick

Application of Impermeable Barriers Combined with Candidate Factor Soaked Beads to Study Inductive Signals in the Chick

DOI:
10.3791/54618-v

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08:04 min

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November 17, 2016

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,
1Randall Division of Cell and Molecular Biophysics,King’s College London, Guy’s Campus

Chapters

  • 00:05Title
  • 00:51Create Barriers from Aluminum Foil
  • 02:08‘Window’ the Incubated Chicken Eggs
  • 03:35Barrier to Prevent Leg Bud Initiation and Bead Operations at Stage 15
  • 05:20Barrier to Prevent Leg Bud Induction and Initiation at Stage 10
  • 06:34Results: Retinoic Acid from the Somites Is Essential for Limb Bud Outgrowth
  • 07:36Conclusion

Summary

Automatic Translation

Automatic Translation

Protocols using impermeable barriers to block induction events between tissues of the main body axis and flank in the chick embryo required for limb formation are described. Beads soaked in candidate inductive signals are used to overcome the effect of barrier placement and analysis of gene expression confirms this.

Tags

Keywords: Impermeable BarrierCandidate FactorLimb InductionChick EmbryoDevelopmental BiologySurgical InterventionSignaling MoleculesLateral Plate MesodermGene ExpressionInductive SignalsLimb Bud InitiationFoil BarrierAir SacEggshellInner Cell Membrane

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