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Empirical, Metagenomic, and Computational Techniques Illuminate the Mechanisms by which Fungicides Compromise Bee Health
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1Vegetable Crop Research Unit,USDA-ARS, 2Department of Entomology,University of Wisconsin-Madison, 3Department of Horticulture,University of Wisconsin-Madison, 4Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias, 5Department of Bacteriology,University of Wisconsin-Madison, 6Laboratory of Genetics,Genome Center of Wisconsin, 7DOE Great Lakes Bioenergy Research Center,Wisconsin Energy Institute, 8J.F. Crow Institute for the Study of Evolution,University of Wisconsin-Madison
Chapters
- 00:05Title
- 01:12Examining the Effect of Fungicide Exposure on Bumble Bee Colony Success Using Field Cage Experiments
- 03:31Examining the Effect of Fungicide Exposure on Microbial Communities in the Pollen-provisions of Bumble Bee Nets Using Laboratory-based In Vivo Trials
- 05:37Results: Effects of Fungicide on Bumble Bee Colonies and Their Microbial Communities
- 07:31Conclusion
Microbial consortia within bumble bee hives enrich and preserve pollen for bee larvae. Using next generation sequencing, along with laboratory and field-based experiments, this manuscript describes protocols used to test the hypothesis that fungicide residues alter the pollen microbiome, and colony demographics, ultimately leading to colony loss.
