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Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis
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Biochemistry
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JoVE Journal Biochemistry
Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis

Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis

DOI:
10.3791/54683-v

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08:55 min

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October 28, 2016

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,
1Institute for Medical Virology,University of Zurich, 2Friedrich Miescher Institute for Biomedical Research

Chapters

  • 00:05Title
  • 00:48Preparation of Cell Lysate for Non-denaturing PAGE
  • 04:04Electrophoresis
  • 04:53Western Blot
  • 06:34Results: Human MxA Protein from IFN-α Stimulated A549 Cells is a Tetramer
  • 07:22Conclusion

Summary

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This article describes a simple and rapid protocol to evaluate the oligomeric state of the dynamin-like GTPase MxA protein from lysates of human cells using a combination of non-denaturing PAGE with western blot analysis.

Tags

Keywords: Multi-subunit Protein ComplexesNon-denaturing Polyacrylamide Gel ElectrophoresisOligomeric StatusA549 CellsVirocellsMxA ProteinCell LysisIodoacetamideDialysisProtein Complex Characterization

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