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Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
JoVE Journal
Genetics
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JoVE Journal Genetics
Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

DOI:
10.3791/54693-v

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10:37 min

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November 30, 2016

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, , , , ,
1Digestive Disorders Unit, National Institute of Nursing Research,National Institutes of Health, DHHS, 2National Institutes of Health Research Scholar,Howard Hughes Medical Institute, 3Internal Medicine, Medical School,University of Michigan

Chapters

  • 00:05Title
  • 00:44Total RNA Purification
  • 02:45miRNA Sample Preparation
  • 05:18miRNA Hybridization
  • 06:47Prep Station and Digital Analyzer
  • 08:28Results: High Throughput Gene Expression of miRNAs in Irritable Bowel Syndrome
  • 09:57Conclusion

Summary

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We describe the use of a multiplexed high-throughput gene expression platform that quantitates gene expression by barcoding and counting molecules in biological substrates without the need for amplification. We used the platform to quantitate microRNA (miRNA) expression in whole blood in subjects with and without irritable bowel syndrome.

Tags

Keywords: MiRNAMicroRNAIrritable Bowel SyndromeGene ExpressionMultiplexHigh-throughputNucleic Acid QuantificationDigital QuantificationBiomarkerWhole BloodRNA ExtractionNormalizationAnnealing Master Mix

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