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JoVE Journal
Genetics

This content is Open Access.

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
 

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Article DOI: 10.3791/54693 10:37 min November 30th, 2016
November 30th, 2016

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Summary

We describe the use of a multiplexed high-throughput gene expression platform that quantitates gene expression by barcoding and counting molecules in biological substrates without the need for amplification. We used the platform to quantitate microRNA (miRNA) expression in whole blood in subjects with and without irritable bowel syndrome.

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Tags

Multiplexed High-throughput Gene Expression Platform Circulating MiRNAs Irritable Bowel Syndrome Nucleic Acid Quantification MicroRNA Expression Digestive Disorders Dysregulated Molecular Mechanisms Biomarker Signatures MicroRNA Quantification Amplification-free Automated RNA Extraction Total RNA Purification BM1 Buffer
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