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Chapters
- 00:05Title
- 01:08Liposome Mediated Transient Transfection
- 02:35Preparing Cell Samples for Staining
- 04:51Extracellular Staining of Cell Samples
- 06:10Intracellular Staining of Cell Samples
- 07:51Data Analysis
- 08:55Results: Simultaneaous Mutations of N-glycosylation Sites Disrupted Cell Surface Expression of CaVα2δ1
- 10:25Conclusion
Inherited cardiac arrhythmias are often caused by mutations that alter the surface delivery of one or more ion channels. Here, we adapt flow cytometry assays to provide a quantification of the relative total and cell surface protein expression of recombinant ion channels expressed in tsA-201 cells.
