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A Buoyancy-based Method of Determining Fat Levels in Drosophila
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A Buoyancy-based Method of Determining Fat Levels in Drosophila

A Buoyancy-based Method of Determining Fat Levels in Drosophila

DOI:
10.3791/54744-v

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08:00 min

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November 01, 2016

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1Department of Medicine, Division of Endocrinology, Metabolism, and Diabetes,University of Colorado Anschutz Medical Campus

Chapters

  • 00:05Title
  • 00:43Collect Eggs from Flies with Genotype of Interest
  • 02:11Transfer Larvae to Experimental Food
  • 03:05Determine Larvae Fat Levels
  • 06:15Results: Neuronal Function in Specific Regions of the Larval Brain Is Necessary and Sufficient for Body Fat Regulation
  • 07:01Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a method to measure organismal fat levels in the third instar (L3) larval stage of Drosophila melanogaster. This method exploits the comparatively low density of fat tissue to differentiate between larvae with altered fat stores. Buoyancy-based analysis is a valuable tool for rapid, reproducible, and economical screening.

Tags

Keywords: DrosophilaFat LevelsBuoyancy-based MethodMetabolismObesityGeneticsLarvaDevelopmental TimelineEgg CollectionYeast PasteFirst Instar LarvaeExperimental Food Vial

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