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JoVE Journal
Biochemistry

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Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System
 

Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System

Article DOI: 10.3791/54782
February 9th, 2017

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Summary February 9th, 2017

We present the setup and experimental procedure to obtain smFRET data from large donor-acceptor networks with a TIRF microscope. The step-by-step analysis of these measurements with the Bayesian inference software Fast-NPS yields high-resolved structural information via the application of adapted dye models.

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