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Immunology and Infection

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SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells

Article DOI: 10.3791/54799-v • 10:24 min • March 3rd, 2017
March 3rd, 2017 •
1, 1, 1, 2,3, 3
1Brown University, 2COBRE Center for Cancer Research, Lifespan Laboratories, Rhode Island and Miriam Hospitals, 3Division of Infectious Diseases, Department of Medicine, Warren Alpert Medical School, Brown University

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Summary
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Here, we describe a quantitative proteomics method using the technique of stable isotope labeling by amino acids in cell culture (SILAC) to analyze the effects of HIV-1 infection on host exosomal proteomes. This protocol can be easily adapted to cells under different stress or infection conditions.

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SILAC Proteomic Characterization Exosomes HIV-1 Infected Cells Exosome Biology External Stresses Proteome Composition Technique Limited Background Proteomics Exosome Biology Disease Studies Bacterial Infections H9 Cell Line Actively Proliferating Stage Test Condition Labeled Medium Stylized FBS C13-labeled L-lysine C13 And N15-labeled L-arginine Unlabeled Medium Doublings Heavy Amino Acids
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