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Immunology and Infection

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SILAC Based protéomique Caractérisation des exosomes du VIH-1 des cellules infectées
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SILAC Based protéomique Caractérisation des exosomes du VIH-1 des cellules infectées

Article DOI: 10.3791/54799-v • 10:24 min • March 3rd, 2017
March 3rd, 2017 •
1, 1, 1, 2,3, 3
1Brown University, 2COBRE Center for Cancer Research, Lifespan Laboratories, Rhode Island and Miriam Hospitals, 3Division of Infectious Diseases, Department of Medicine, Warren Alpert Medical School, Brown University

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Ici, nous décrivons une méthode de protéomique quantitative en utilisant la technique de marquage isotopique stable par les acides aminés dans la culture cellulaire (SILAC) pour analyser les effets du VIH-1 infection sur protéomes exosomales hôte. Ce protocole peut être facilement adapté à des cellules sous différentes contraintes ou infection conditions.

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Infection numéro 121 exosomes extracellulaires Véhicules le VIH-1 protéomique spectrométrie de masse SILAC Bioinformatique
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