Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Developmental Biology

This content is Open Access.

Analysis of Coronary Vessels in Cleared Embryonic Hearts
 

Analysis of Coronary Vessels in Cleared Embryonic Hearts

Article doi: 10.3791/54800
December 7th, 2016

Summary December 7th, 2016

We present a protocol for the analysis of coronary vessels in whole embryonic murine hearts up to E15.5, using standard immunological staining methods followed by optical clearance and confocal microscopy. This technique enables visualization of blood vessels throughout the entire heart without the need for time-consuming analysis of serial sections.

Transcript

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter