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A Method for Extracting Pigments from Squid Doryteuthis pealeii

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Biology

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JoVE Journal Biology

A Method for Extracting Pigments from Squid Doryteuthis pealeii

A Method for Extracting Pigments from Squid Doryteuthis pealeii

DOI:

10.3791/54803-v

•

11:03 min

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November 09, 2016

•

Christopher W. DiBona, Thomas L. Williams, Sean R. Dinneen, Stephanie F. Jones Labadie, Leila F. Deravi²

¹Department of Chemistry,University of New Hampshire, ²Materials Science Program,University of New Hampshire

Chapters

00:05Title
01:08Doryteuthis pealii Dissection
02:30Isolating Chromatophore Pigment Granules
05:25Pigment Extraction
06:45Scanning Electron Microscopy (SEM) and Spectrophotometric Analysis (UV-Vis)
08:28Results: Successful Pigment Extraction Is Confirmed by UV-Vis Spectra and Decreased Granule Size
09:44Conclusion

Summary

Automatic Translation

A protocol for the extraction of pigments from the nanostructured granules in squid Doryteuthis pealeii chromatophores is presented.

Tags

Keywords: Pigment Extraction Squid Doryteuthis Pealeii Chromatophores Nano-structured Granules Cephalopod Biology Color Change Dissection Centrifugation Papain Collagenase

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