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JoVE Journal
Developmental Biology

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Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish
 

Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish

Article doi: 10.3791/54820
December 21st, 2016

Summary December 21st, 2016

The interrenal gland in zebrafish is the teleostean counterpart of the mammalian adrenal gland. This protocol introduces how to perform the 3-β-hydroxysteroid dehydrogenase (Δ5-4 isomerase; 3β-Hsd) enzymatic activity assay, which detects differentiated steroidogenic cells in the developing zebrafish.

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