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JoVE Journal
Immunology and Infection

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Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro
 

Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro

Article DOI: 10.3791/54826 10:05 min January 25th, 2017
January 25th, 2017

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Summary

We describe here a method for obtaining and identifying a newly characterized subpopulation of neutrophil-derived giant phagocytes. These cells develop in culture from fresh human blood neutrophils, and are characterized by phagocytosis, autophagy, immensely large size, and extended lifespan. This method is essential to further investigate this unique neutrophil-derived subpopulation.

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Development Identification Novel Subpopulation Human Neutrophil-derived Giant Phagocytes In Vitro Inflammatory Anti-inflammatory Immune Responses Long-term Culture Conditions Significance Functions Activation Plasticity Research Assistant Post Doc Sterile Scalp Vein Set Venous Blood Biosafety Laminar Flow Hood Dilute Aliquots Whole Blood Sample ION-free PBS Heat Inactivated FCS Polysucrose 1119 Polysucrose 1077 Polypropylene Conical Centrifuge Tube
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