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Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro

Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro

DOI:
10.3791/54826-v

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10:05 min

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January 25, 2017

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, , , ,
1The Lloyd Rigler Sleep Apnea Research Laboratory, Unit of Anatomy and Cell Biology, The Ruth and Bruce Rappaport Faculty of Medicine,Technion-Israel Insitute of Technology

Chapters

  • 00:05Title
  • 00:59Neutrophil Isolation and Giant Phagocyte (Gphi) In Vitro Development
  • 04:03Confocal Laser Scanning Microscopy
  • 07:08Results: Representative Gphi Characterization
  • 08:50Conclusion

Summary

Automatic Translation

Automatic Translation

We describe here a method for obtaining and identifying a newly characterized subpopulation of neutrophil-derived giant phagocytes. These cells develop in culture from fresh human blood neutrophils, and are characterized by phagocytosis, autophagy, immensely large size, and extended lifespan. This method is essential to further investigate this unique neutrophil-derived subpopulation.

Tags

Keywords: Human NeutrophilsGiant PhagocytesIn Vitro CultureInflammatory ResponseAntiinflammatory ResponseDensity Gradient SeparationPolymorphonuclear CellsPMNCell IsolationCell Culture

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