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Immunology and Infection
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Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291
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Immunology and Infection
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JoVE Journal Immunology and Infection
Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

DOI:
10.3791/54850-v

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06:51 min

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December 10, 2016

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, , ,
1Department of Population Health and Pathobiology,North Carolina State University College of Veterinary Medicine, 2Department of Pathology and Laboratory Medicine, Lineberger Comprehensive Cancer Center,University of North Carolina School of Medicine

Chapters

  • 00:05Title
  • 00:53Inoculation of Mice with Clostridium difficile
  • 03:22Enumeration of C. difficile from Mouse Fecal Content
  • 05:03Results: The Murine Model of C. difficile Infection Demonstrates Significant Weight Loss, Bacterial Colonization and Histopathological Changes in Infected Mice
  • 06:10Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol outlines the cefoperazone mouse model of Clostridium difficile infection (CDI) using a clinically-relevant and genetically-tractable strain, R20291. Emphasis on clinical disease monitoring, C. difficile bacterial enumeration, toxin cytotoxicity, and histopathological changes throughout CDI in a mouse model are detailed in the protocol.

Tags

Keywords: Clostridium DifficileMouse ModelCefoperazoneSpore InoculumGavageAnaerobicDilutionEnumerationToxin CytotoxicityHistopathology

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