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JoVE Journal
Immunology and Infection

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Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291
 

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Article DOI: 10.3791/54850
December 10th, 2016

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Summary December 10th, 2016

This protocol outlines the cefoperazone mouse model of Clostridium difficile infection (CDI) using a clinically-relevant and genetically-tractable strain, R20291. Emphasis on clinical disease monitoring, C. difficile bacterial enumeration, toxin cytotoxicity, and histopathological changes throughout CDI in a mouse model are detailed in the protocol.

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