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Biochemistry

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Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline

Article DOI: 10.3791/54861-v • 11:09 min • January 5th, 2017
January 5th, 2017 •
1, 2,3, 4
1Structural Biology Group, European Synchrotron Radiation Facility, 2European Molecular Biology Laboratory, 3School of Chemical and Physical Sciences, Keele University, 4Groupe de Microscopie Electronique et Méthodes, Institut de Biologie Structurale

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Summary
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The determination of the solution structure of a protein by small angle X-ray scattering (SAXS) requires monodisperse samples. Here, we present two possibilities to ensure minimal delays between sample preparation and data acquisition: online size-exclusion chromatography (SEC) and online ion-exchange chromatography (IEC).

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Online Size-exclusion Ion-exchange Chromatography SAXS Beamline Low Resolution Model Data Sample Preparation Data Acquisition Structural Biology Radius Of Gyration Intra-particular Distance Particle Shape Folding Denaturation Disorder Molar Dispersed Samples Sample Denaturation SEC Test Runs Gel Filtration Buffer HPLC System Flow Rate Column Pressure Acquisition Time Size Exclusion Chromatography Column Sample Changer Mode
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