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JoVE Journal
Biochemistry

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Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline
 

Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline

Article DOI: 10.3791/54861
January 5th, 2017

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Summary January 5th, 2017

The determination of the solution structure of a protein by small angle X-ray scattering (SAXS) requires monodisperse samples. Here, we present two possibilities to ensure minimal delays between sample preparation and data acquisition: online size-exclusion chromatography (SEC) and online ion-exchange chromatography (IEC).

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