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Immunology and Infection
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Optimization of a Quantitative Micro-neutralization Assay
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Optimization of a Quantitative Micro-neutralization Assay

Optimization of a Quantitative Micro-neutralization Assay

DOI:
10.3791/54897-v

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10:09 min

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December 14, 2016

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, ,
1Mill Hill Laboratory,The Francis Crick Institute

Chapters

  • 00:05Title
  • 00:59Virus Titration
  • 04:25Titration Quantitation
  • 05:59Virus Neutralization
  • 07:20Neutralization Quantification
  • 08:04Results: Micro-neutralization Optimization
  • 09:34Conclusion

Summary

Automatic Translation

Automatic Translation

This study describes an imaging-based micro-neutralization assay to analyze the antigenic relationships between viruses. The protocol employs a flatbed scanner and has four steps, including titration, titration quantitation, neutralization, and neutralization quantitation. The assay works well with current circulating influenza A(H1N1)pdm09, A(H3N2), and B viruses.

Tags

Keywords: Quantitative Micro-neutralization AssayInfluenza VirusesAntibody CharacterizationAntiviral ActivityBSL 2BSL 3+Virus Growth Medium (VGM)Serial DilutionsVirus InfectionOverlayPFA FixationPermeabilizationWHO Collaborating Center For Influenza

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