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April 11, 2017
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Summary
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A number of FRET-based force biosensors have recently been developed, enabling the protein-specific resolution of intracellular force. In this protocol, we demonstrate how one of these sensors, designed for the linker of the nucleoskeleton-cytoskeleton (LINC) complex protein Nesprin-2G can be used to measure actomyosin forces on the nuclear LINC complex.
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Cite this Article
Arsenovic, P. T., Bathula, K., Conway, D. E. A Protocol for Using Förster Resonance Energy Transfer (FRET)-force Biosensors to Measure Mechanical Forces across the Nuclear LINC Complex. J. Vis. Exp. (122), e54902, doi:10.3791/54902 (2017).
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