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Bioengineering
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2D and 3D Matrices to Study Linear Invadosome Formation and Activity
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2D and 3D Matrices to Study Linear Invadosome Formation and Activity

Article DOI: 10.3791/54911-v • 12:26 min • June 2nd, 2017
June 2nd, 2017 •
*1,2, *1, 1,3,4, 2, 2, 1, 1
1INSERM U1053, 2Icahn School of Medicine at Mount Sinai, Tisch Cancer Institute, 3Faculté de Médecine, Casablanca, Université Mohammed 6 des Sciences de la Santé (UM6SS), 4Laboratoire National de Référence
* These authors contributed equally

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Summary
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This protocol describes how to prepare a 2D mixed matrix, consisting of gelatin and collagen I, and a 3D collagen I plug to study linear invadosomes. These protocols allow for the study of linear invadosome formation, matrix degradation activity, and the invasion capabilities of primary cells and cancer cell lines.

Tags

2D Matrices 3D Matrices Linear Invadosome Formation Invadosome Activity Cell And Extracellular Matrix Interaction Matrix Degradation Physiological Processes Pathological Processes Neurogenesis Cell Tumor Invasion Actin-based Structure Invadosomes Podosomes Invadopodia Self-organization Degradation Shapes Dots Aggregates Rosettes Metalloproteinases MMP2 MT1-MMP Type 1 Collagen Fibrils Zymography In-situ Assay Mixed Matrix Gelatin Type 1 Collagen Fibrils Impact Of Type 1 Collagen Fibrils On Invadosomes Matrix Generation Process
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