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JoVE Journal Genetics
Ingegneria fattori artificiali per manipolare in modo specifico l'alternanza di splicing nelle cellule umane
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Ingegneria fattori artificiali per manipolare in modo specifico l'alternanza di splicing nelle cellule umane

Article DOI: 10.3791/54967-v • 10:06 min • April 26th, 2017
April 26th, 2017 •
*1, *1, 2, 1, 1, 1, 1
1Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences (SIBS), 2Institute of Cancer Stem Cell, Second Affiliated Hospital, Cancer Center, Dalian Medical University
* These authors contributed equally

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Questa relazione descrive un metodo bioingegneria per progettare e costruire nuovi fattori artificiali splicing (ASF) che specificamente modulano la giunzione di geni bersaglio in cellule di mammifero. Questo metodo può essere ulteriormente ampliata per progettare vari fattori artificiali di manipolare altri aspetti del metabolismo dell'RNA.

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Genetica RNA fattori di legame artificiale ingegneria delle proteine fattori di splicing splicing alternativo impalcatura PUF RNA proteine ​​leganti il cancro
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