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JoVE Journal Genetics
Criação e Double-stranded mediada por RNA silenciamento de genes na Esconder Beetle,
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Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis
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Criação e Double-stranded mediada por RNA silenciamento de genes na Esconder Beetle,

Article DOI: 10.3791/54976 • 09:57 min
December 28th, 2016 •
1,2, 1, 1,2
1Entomology Department, University of Maryland, 2Program in Molecular and Cell Biology, University of Maryland

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December 28th, 2016

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Aqui, apresentamos os protocolos de criação de um besouro intermediário de germes, Dermestes maculatus (D. maculatus) no laboratório. Também compartilhamos protocolos de RNAi embrionário e parental e métodos para análise de fenótipos embrionárias para estudar a função de genes nesta espécie.

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