Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / In Vitro Differentiation of Human CD4 + FOXP3…
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.

JoVE Journal Immunology and Infection
In Vitro Differentiation of Human CD4 + FOXP3 cellules + Induced T régulatrices (de iTregs) de Naïf cellules T CD4 + en utilisant un protocole TGF-β contenant
Analysis of Human Natural Killer Cell Metabolism
Journal (Immunology and Infection)
Analysis of Human Natural Killer Cell Metabolism
Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation
Journal (Immunology and Infection)
Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation
 
Click here for the English version

In Vitro Differentiation of Human CD4 + FOXP3 cellules + Induced T régulatrices (de iTregs) de Naïf cellules T CD4 + en utilisant un protocole TGF-β contenant

Article DOI: 10.3791/55015-v • 08:20 min • December 30th, 2016
December 30th, 2016 •
1, *1, *1, 1
1Unit of Computational Medicine, Center for Molecular Medicine, Department of Medicine Solna, Karolinska Institutet, Karolinska University Hospital, & Science for Life Laboratory
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Ce protocole décrit la génération reproductible et phénotypage des cellules T régulatrices humaines induites (les iTregs) à partir de cellules T CD4 + naïves in vitro. Différents protocoles pour FOXP3 induction permettent l'étude des phénotypes iTreg spécifiques obtenus avec les protocoles respectifs.

Tags

Immunologie numéro 118 les cellules T régulatrices Treg les cellules T CD4 + l'isolement cellulaire magnétique, des cytokines FOXP3 le TGF-β l'IL-2 intracellulaire cytométrie de flux de qRT-PCR
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter