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Chapters
- 00:05Title
- 00:52Prepare Solutions and Bacteria Culture for NGT-3D and NGB-3D
- 02:23Making NGT-3D and NGB-3D
- 05:18Measure Fitness of Worm Population on NGT-3D
- 06:47Results: Development, Fertility, and Lifespan of C. elegans Cultivated in NGT-3D Is Comparable with That of the Standard 2D NGM
- 08:12Conclusion
We present a simple method to construct 3D nematode cultivation systems called NGT-3D and NGB-3D. These can be used to study nematode fitness and behaviors in habitats that are more similar to natural Caenorhabditis elegans habitats than the standard 2D laboratory C. elegans culture plates.
