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A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
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Biology
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JoVE Journal Biology
A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix

DOI:
10.3791/55051-v

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09:40 min

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January 04, 2017

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, ,
1School of Biochemistry,University of Bristol

Chapters

  • 00:05Title
  • 00:56Removal of Cells with Ammonium Hydroxide Solution
  • 03:21Sterile Preparation of Cell-derived Extracellular Matrix for Cell Functional Assays
  • 05:01Isolation of Extracellular Matrix for SDS-PAGE, Immunoblot Analysis, or Proteomics
  • 06:30Results: Isolation and Analysis of Cell-derived Extracellular Matrix
  • 08:26Conclusion

Summary

Automatic Translation

Automatic Translation

The extracellular matrix plays a major role in defining the microenvironment of cells and in modulating cell behavior and phenotype. We describe a rapid method for the isolation of cell-derived extracellular matrix, which can be adapted to different scales for microscopic, biochemical, proteomic, or functional studies.

Tags

Keywords: Extracellular MatrixCell-derived ECMMatrix IsolationFunctional StudiesFluorescence MicroscopyAmmonium HydroxideCell LysisDeionized Water WashesScalable MethodAdherent CellsECM CompositionECM OrganizationECM Interactions

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