A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)&#8211;Cell Interaction and the Resultant Bioeffects at the Single-cell Level

Fenfang Li; Fang Yuan; Georgy Sankin; Chen Yang; Pei Zhong

doi:10.3791/55106

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A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level

11,708 Views

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11:14 min

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January 10, 2017

DOI:

10.3791/55106-v

DOI:

10.3791/55106-v

•

11:14 min

January 10, 2017

•

11686 Views

Fenfang Li¹, Fang Yuan², Georgy Sankin¹, Chen Yang¹, Pei Zhong¹

¹Mechanical Engineering and Materials Science,Duke University ²Huacells Corp

Summary

A microfluidic chip was fabricated to produce pairs of gold dots for tandem bubble generation and fibronectin-coated islands for single-cell patterning nearby. The resultant flow field was characterized by particle image velocimetry and was employed to study various bioeffects, including cell membrane poration, membrane deformation, and intracellular calcium response.