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JoVE Journal
Immunology and Infection

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Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator
 

Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator

Article DOI: 10.3791/55107
April 5th, 2017

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Summary April 5th, 2017

This manuscript describes a simple method to measure the phagosomal pH and area as well as the cytoplasmic pH of human and mouse neutrophils using the ratiometric indicator seminaphthorhodafluor (SNARF)-1, or S-1. This is achieved using live-cell confocal fluorescence microscopy and image analysis.

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