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Genetics

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Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions
 

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions

Article DOI: 10.3791/55112
December 28th, 2016

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Summary December 28th, 2016

Here, we present human cell culture protocols to analyze translation initiation factors that bind the 5' cap of mRNA during physiological oxygen conditions. This method utilizes an Agarose-linked m7GTP cap analog and is suitable to investigate cap-binding factors and their interacting partners.

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