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JoVE Journal
Developmental Biology

This content is Open Access.

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps
 

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

Article doi: 10.3791/55127
February 9th, 2017

Summary February 9th, 2017

The segmentation clock drives oscillatory gene expression across the pre-somitic mesoderm (PSM). Dynamic Notch activity is key to this process. We use imaging and computational analyses to extract temporal dynamics from spatial expression data to demonstrate that Delta ligand and Notch receptor expression oscillate in the vertebrate PSM.

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