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Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps
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Chapters
- 00:05Title
- 00:50PSM Explant Dissection
- 02:49Immunohistochemistry of PSM Explants
- 04:38Fluorescent In Situ Hybridization (FISH) of PSM Explants
- 07:09Prepare Samples for Imaging
- 08:33Results: Spatiotemporal Expression Profiles for Dll1 and Notch1 According to Expression of the Clock Gene Lfng
- 10:21Conclusion
The segmentation clock drives oscillatory gene expression across the pre-somitic mesoderm (PSM). Dynamic Notch activity is key to this process. We use imaging and computational analyses to extract temporal dynamics from spatial expression data to demonstrate that Delta ligand and Notch receptor expression oscillate in the vertebrate PSM.
