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In Utero Elektroporatie benaderingen om de prikkelbaarheid van neuronale subpopulaties en Single-cell Connectivity Studie
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In Utero Elektroporatie benaderingen om de prikkelbaarheid van neuronale subpopulaties en Single-cell Connectivity Studie

Article DOI: 10.3791/55139-v • 10:49 min • February 15th, 2017
February 15th, 2017 •
1, 2, 2, 1
1Department for Molecular and Cellular Biology, Centro Nacional de Biotecnología (CNB-CSIC), 2Molecular Neurobiology Department, Centro de Biología Molecular "Severo Ochoa", Consejo Superior de Investigaciones Científicas (CSIC-UAM)

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Dit manuscript biedt protocollen die gebruik maken van in de baarmoeder elektroporatie (IUE) om de structurele connectiviteit van neuronen te beschrijven op het single-cell niveau en de prikkelbaarheid van fluorescent gelabelde neuronen. Histologie wordt gebruikt dendritische en axonale uitsteeksels karakteriseren. Whole-cell opname in acute plakjes wordt gebruikt om prikkelbaarheid te onderzoeken.

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Neuroscience hersenen ontwikkeling cortex elektrofysiologie corpus callosum elektroporatie
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