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Biochemistry

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Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
 

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Article DOI: 10.3791/55148
March 5th, 2017

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Summary March 5th, 2017

Many proteins perform their function when attached to membrane surfaces. The binding of extrinsic proteins on nanodisc membranes can be indirectly imaged by transmission electron microscopy. We show that the characteristic stacking (rouleau) of nanodiscs induced by the negative stain sodium phosphotungstate is prevented by the binding of extrinsic protein.

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